Elisabetta Mereu
- f502-c003 Dottorato in Medicina Molecolare
- Dottorato: 27° ciclo
- Matricola: 320394
Tesi di dottorato
Research Activity 2014-2015
Anaplastic Large Cell Lymphoma (ALCL) is a clinical and biological heterogeneous disease including systemic ALK positive and ALK negative entities. To discover biomarkers and/or genes involved in ALK negative ALCL pathogenesis, we applied the Cancer Outlier Profile Analysis (COPA) algorithm to a gene expression profiling data set including 249 cases of T-cell non-Hodgkin lymphoma and normal T-cells. Ectopic co-expression of ERBB4 and COL29A1 genes was detected in 24% of ALK negative ALCL patients. RNA sequencing and 5'RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE) identified two novel ERBB4 truncated transcripts, displaying intronic Transcription Start Sites. By luciferase assays we defined that the expression of ERBB4 aberrant transcripts is promoted by endogenous intronic Long Terminal Repeats (LTRs). ERBB4 expression was confirmed at protein level by western blotting and immunohistochemistry. Finally, we demonstrated that the expression of ERBB4 truncated forms show oncogenic potentials and that ERBB4 pharmacological inhibition partially controls ALCL cell growth and disease progression in an ERBB4 positive patient-derived tumorgraft model. In conclusion, we identified a new subclass of ALK negative ALCL characterized by aberrant expression of ERBB4 truncated transcripts carrying intronic 5'UTRs.
Attività di ricerca
2015
“Identification of a new subclass of ALK negative ALCL expressing aberrant levels of ERBB4". Scarfò I, Pellegrino E, Mereu E, Kwee I, Agnelli L, Bergaggio E, Garaffo G, Vitale N, Caputo M, Machiorlatti R, Circosta P, Abate F, Barreca A, Novero D, Mathew S, Rinaldi A, Tiacci E, Serra S, Deaglio S, Neri A, Falini B, Rabadan R, Bertoni F, Inghirami G, Piva R. Blood. 2015 Oct 13. pii: blood-2014-12-614503.
2014
“The krüppel-like factor 2 transcription factor gene is recurrently mutated in splenic marginal zone lymphoma”. Piva R, Deaglio S, Famà R, Buonincontri R, Scarfò I, Bruscaggin A, Mereu E, Serra S, Spina V, Brusa D, Garaffo G, Monti S, Dal Bo M, Marasca R, Arcaini L, Neri A, Gattei V, Paulli M, Tiacci E, Bertoni F, Pileri SA, Foà R, Inghirami G, Gaidano G, Rossi D. Leukemia. 2014 Oct 6
2012
"Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma". Agnelli L, Mereu E, Pellegrino E, Limongi T, Kwee I, Bergaggio E, Ponzoni M, Zamò A, Iqbal J, Piccaluga PP, Neri A, Chan WC, Pileri S, Bertoni F, Inghirami G, Piva R; European T-Cell Lymphoma Study Group. Blood. 2012 Aug 9;120(6):1274-81.
2015
SIBBM, Frontiers in molecular byology
1-3 Luglio 2015
2014
Pier Paolo Pandolfi
Ciclo di seminari 20-23 maggio 2014
23 maggio 2014, Ospite: Epigen
Chromatin seminar - Turin
27 giugno 2014,
Workshop “Targeted Therapy of Cancer: where we are heading"- Torino
4-5 ottobre 2014
2014 International Conference Molecular Clinical Oncology “Precision Medicine”