Anna Luganini
- f403-c403 Dottore in Medicina Molecolare
- Dottorato: 25° ciclo
- Matricola: 276323
Contatti
- anna.luganini@unito.it
- indirizzo in Immunologia e Biologia Cellulare
- https://dott-mm.campusnet.unito.it/do/studenti.pl/Show?276323
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Supervisore
Santo LandolfoAttività di ricerca
Dr. Anna Luganini is engaged in research activities at the Laboratory of Microbiology and Virology (LMV), Department of Life Sciences and Systems Biology, University of Turin. Since 2004 she has focused her attention primarily in the study of the molecular mechanisms of the interactions between Cytomegalovirus and host cells, the development of new antiviral agents, as well as the characterization of functional and biological properties of the interferon system in the context of viral replication. These studies were developed through the analysis of viral replication strategies in cells of different origin and in different physiologic conditions, as well as through the characterization of new viral proteins. In particular, she has carried out research activities that have been articulated on the following topics:
•Production and characterization of fully human monoclonal antibodies able to neutralize the HCMV infection.
•Production and characterization of new indicator cell lines for the detection of HCMV infectious particles and for searching new antiviral molecules against the IE2 protein activities.
•Characterization of antiviral activity of new peptide-derivatized dendrimers.
•Characterization of the anti-cytomegalic activity of the IFN-inducible human protein IFI16. Study of its effects on viral replication in fibroblasts and endothelial cells. Generation and validation of recombinant adenoviral vectors for high efficiency IFI16 expression in primary cells.
•Study of molecular and physiological alterations in endothelial cells following HCMV infection.
•Using the bacmid technology, she studied the role of cellular transcription factors NF-kB, Elk-1and SRF in the context of the replicative cycle of HCMV in cells of different origin and in different physiologic conditions.
•Study of the functional role of the US12 gene family in the replication of HCMV in endothelial and epithelial cells.
1. Caposio P., Musso T., Luganini A., Inoue H., Gariglio M., Landolfo S., and Gribaudo G.
“Targeting the NF-kB pathway through pharmacological inhibition of IKK2 prevents human cytomegalovirus replication and virus-induced inflammatory response in infected endothelial cells”.
Antiviral Res., 73: 175-184, 2007. (IF 4.439).
2. Caposio P., Luganini A., Hahn, G., Landolfo S., and Gribaudo G.
“Activation of the virus-induced NF-kB/IKK signaling axis is critical for the replication of human cytomegalovirus in quiescent cells”. Cell. Microbiol., 9: 2040-2054, 2007. (IF 5.458).
3. Luganini A., Caposio P., Landolfo S., and Gribaudo G.
“Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry”. Antimicrob. Agents Chemother, 52: 1111-1120, 2008. (IF 4.672).
4. Luganini A., Caposio P., Mondini M., Landolfo S., and Gribaudo G.
“New cell-based indicator assays for the detection of human cytomegalovirus infection and
screening of inhibitors of viral immediate-early 2 (IE2) protein activity”. J. Applied Microbiol., 1791-1801, 2008. (IF 2.337).
5. Funaro A., Gribaudo G., Luganini A., Ortolani, E., Lo Buono N., Murphy M., Vicenzi, E., Cassetta, L., Landolfo S.,Garotta G., and Malavasi F.
“Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells”. BMC Biotechnol. 8: 85, 2008. (IF 2.35).
6. Luganini A., Giuliani A., Pirri G., Pizzuto L., Landolfo S., and Gribaudo G.
“Peptide-derivatized dendrimers inhibit human cytomegalovirus infection by blocking virus binding to cell surface heparan sulphate”. Antiviral Res., 85:532-540, 2010. (IF 4.439).
7. Caposio P.*, Luganini A.*., Bronzini M., Landolfo S., and Gribaudo G.
“The Elk-1 and serum response factor binding sites in the major immediate-early promoter of human cytomegalovirus are required for efficient viral replication in quiescent cells and compensate for inactivation of the NF-kappaB sites in proliferating cells”. J Virol., 84:4481-4493, 2010.
*Equal contribution.
8. Fiorentini S., Luganini A., Dell’Oste V., Lorusso B., Cervi E., Caccuri F., Bonardelli S.,
Landolfo S., Caruso A., and Gribaudo G.
“Human cytomegalovirus productively infects lymphatic endothelial cells and induces a secretome that promotes angiogenesis and lymphangiogenesis through IL-6 and GM-CSF”. J Gen. Virol., 92:650-660, 2010. (IF 3.568).
9. Luganini A., Nicoletto S.F., Pizzuto L., Pirri G., Giuliani A., Landolfo S., and Gribaudo G. “Inhibition of herpes simplex virus type 1 and type 2 infections by peptide-derivatized dendrimers”. Antimicrob Agents Chemother. 55, 3231-3239, 2011. (IF 4.672).
10. Gariano G.R., Dell’Oste V., Bronzini M., Gatti D., Luganini A., De Andrea M., Gribaudo G., Gariglio M., and Landolfo S.
“The intracellular DNA sensor IFI16 gene acts as restriction factor for human cytomegalovirus replication”. PLoS Pathog., 8, e1002498, 2012. (IF 9.079).
11. Bronzini M.*, Luganini A.*, Dell'Oste V., De Andrea M., Landolfo S., and Gribaudo G.
“The US16 gene of Human Cytomegalovirus is required for efficient viral infection of endothelial and epithelial Cells”. J Virol., Jun;86(12):6875-88., 2012. (IF 5.189).
*Equal contribution.
12. Mercorelli B., Luganini A., Muratore G., Massari S., Terlizzi M.E., Tabarrini O., Landolfo S., Gribaudo G., Palù G., and Loregian A.
“The analysis of the inhibition of human cytomegalovirus IE2 protein by the 6-aminoquinolone WC5 reveals interference with DNA-binding activity of the protein and new target sites on a responsive promoter”. J Virol, 2013 submitted. (IF 4.893).
13. Del Prete A.*, Luganini A.*, Scutera S., Rossi S., Anselmo A., Badolato R., Landolfo S., Sozzani S., Gribaudo G., and Musso T.
“IFN-α production by plasmacytoid dendritic cells is dispensable for an effective anti-cytomegalovirus response in AP-3 deficient mice”.J Interferon Cytokine Res, 2013 submitted. (IF 3.297).
*Equal contribution.
1. Caposio P., Luganini A., Hahn G., Landolfo S., and Gribaudo G.
“The Human Cytomegalovirus 46 kDa UL72 protein is not an active dUTPase but a late protein dispensable for replication in fibroblasts”.6° Convegno FISV, Riva del Garda, 403, 2004.
2. Caposio P., Luganini A., Liofante L., Landolfo S., and Gribaudo G.
“Human Cytomegalovirus stimulates cyclooxygenase-2 gene expression in endothelial cells”.
7° Convegno FISV, Riva del Garda, D5.1, 2005.
3. Caposio P., Luganini A., Landolfo S., and Gribaudo G.
“Activation of the NF-kB signaling axis is required for human cytomegalovirus productive replication”.25° Congresso Nazionale SIMGBM, Orvieto, 33, 2006.
4. Caposio P., Luganini A., Hahn G., Landolfo S., and Gribaudo G.
“Activation of the virus-induced IKK/NF-kB signaling axis is critical for the replication of HCMV in quiescent cells”.11th International CMV and Betaherpes Virus Workshop, Toulouse, S2-P9, 2007.
5. Caposio P., Luganini A., Hahn G., Landolfo S., and Gribaudo G.
“Mutational analysis of human cytomegalovirus MIEP reveals a critical role of the NF-kB binding sites for the virus replication in quiescent cells”.13th International Conference on Immunology and Prophylaxis of Human Herpesvirus Infections, Orvieto, 2007.
6. Luganini A., Caposio P., Landolfo S., and Gribaudo G.
“Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry”.8th National Congress of the Italian Society of Virology, Orvieto, 2008.
7. Caposio P., Jarvis M., Ashlee M., Streblow D., Luganini A., Bronzini M., Landolfo S., Gribaudo G., and Nelson J.“A Human Cytomegalovirus late-kinetic class of gene(s) induces the production of factors in the cellular secretome that promotes endothelial cell angiogenesis and prevents cellular apoptosis”.9th National Congress of the Italian Society of Virology, Orvieto, 2009.
8. Luganini A., Caposio P., Mondini M., Landolfo S., and Gribaudo G. “New cell-based indicator assays for the detection of human cytomegalovirus infection and screening of inhibitors of viral immediate early 2 (IE2) protein activity.”9th National Congress of the Italian Society of Virology, Orvieto, 2009.
9. Caposio P., Luganini A., Bronzini M., Landolfo S., and Gribaudo G.
“The Elk-1 and serum response factor sites in the major immediate-early promoter of human cytomegalovirus are required for efficient viral replication in quiescent cells”.37° Congresso Nazionale SIM, Torino, 2009.
10. Caposio P., Luganini A., Bronzini M., Landolfo S., and Gribaudo G.
“The ELK-1 and serum response factor binding sites in the major immediate early promoter of the human citomegalovirus are required for efficient viral replication in quiescent cells”.4th European Congress of Virology, Cernobbio, 2010.
11. De Andrea M., Luganini A., Gugliesi F., Gribaudo G., Gariglio M., and Landolfo S.
“The interferon-inducible IFI16 gene acts as a restriction factor for human citomegalovirus replication”. 8th Joint Conference of the International Cytokine Society and the International Society for Interferon and Cytokine Research, Chicago, 79, 2010.
12. Fiorentini S., Caposio P., Luganini A., Garrafa E., Caruso A., and Gribaudo G.
“Human cytomegalovirus productively infects lymphatic endothelial cells and triggers a secretome that promotes angiogenesis and lymphangiogenesis”.35th Annual International Herpesvirus Workshop, Salt Lake City, USA, July 24-29 2010, p.2.36-2,36
13. Dell’Oste V., De Andrea M., Luganini A., Gariano G.R., Gatti D., Bronzini M., Gribaudo G., Gariglio M., and Landolfo S.
“The interferon-inducible IFI-16 gene is a restriction factor for Human Cytomegalovirus replication”.13th International CMV/BetaHerpesvirus Workshop, Norimberga (Germany), 97, 2011.
14. Luganini A., Bronzini M., Dell’Oste V., Landolfo S., and Gribaudo G.
“The US16 gene of human cytomegalovirus encodes a tropism factor required for infection of endothelial and epithelial cells”.29° Congresso Nazionale SIMGBM, Pisa, 2011.
15. Luganini A., Nicoletto S.F., Pizzuto L., Pirri G., Giuliani A., Landolfo S., and Gribaudo G. “Inhibition of herpes simplex virus type 1 and type 2 infections by peptide-derivatized dendrimers”.
Innovative Approaches for identification of Antiviral Agents Summer School, Santa Margherita di Pula (CA), Italy, 2012.
16. Gatti D., Dell’Oste V., Gugliesi F., Luganini A., De Andrea M., Gribaudo G., Gariglio M., and Landolfo S. “Il citomegalovirus umano induce la delocalizzazione della proteina interferon-inducibile IFI 16 dal nucleo al citoplasma”. 40° Congresso Nazionale SIM, Riccione, 2012.
17. Dell’Oste V., Gatti D., Gugliesi F., Luganini A., De Andrea M., Gribaudo G., Gariglio M., and Landolfo S. “La proteina interferon inducibile IFI 16: fattore di restrizione per la replicazione degli herpes virus umani”.40° Congresso Nazionale SIM, Riccione, 2012.
18. Luganini A., Terlizzi M.E., Cavaletto N., Olivero C., and Gribaudo G.
“Functional analysis of the US12 gene family of human cytomegalovirus (HCMV) reveals the presence of multiple endothelial cell tropism determinants”.38th Annual International Herpesvirus Workshop, Grand Rapids, Michigan, USA. 1.24-p54, 2013.
19. Terlizzi M.E., Luganini A., Cavaletto N., Olivero C. and Gribaudo G.
“US16 protein of Human Cytomegalovirus regulates viral entry in endothelial and epithelial cells”
30° Congresso Nazionale SIMGBM, Ischia, 2013.