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Dott. Marco Macagno

  • Dottorato: 24° ciclo
  • Matricola: 294308

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Tesi di dottorato

Innate immunosurveillance in ERB-2 autochthonous carcinogenesis in transgenic mice

Tutor: Federica Cavallo

Data discussione tesi: 28/01/2013

 

PERFORIN-DEPENDENT IMMUNE SURVEILLANCE AGAINST Her-2/neu CARCINOMAS IN TRANSGENIC MALE AND FEMALE MICE

Tumor immunesurveillance rests on T cell and NK cells. In both cases perforin (pfp) may be a major effector molecule. Our previous works showed that pfp is involved in the  immunosurveillance of ErbB-2-mammary carcinogenesis in BALB/c female mice transgenic for the transforming rat ErbB-2(neu) oncogene under the control of the mouse mammary tumor virus promoter (BALB-neuT mice) (Street et al, 2007; Cancer Res 67:5454). Here we evaluate the unique role of pfp in male mammary cancer immunosurveillance. While BALB-neuT male mice rarely develop mammary carcinomas, all BALB-neuT females develop multiple, fast-growing, invasive carcinomas in all mammary glands. When we back-crossed BALB-neuT mice with pfpKO BALB/c mice, tumor onset in male and female BALB-neuT and BALB-neuT/pfpKO mice was evaluated. An accelerated onset of mammary carcinomas and a higher number of carcinomas per mouse were found in BALB-neuT/pfpKO female mice. Moreover, while BALB-neuT male mice rarely develop mammary carcinomas, surprisingly, the great majority of BALB-neuT-pfpKO male mice develops them. BALB-neuT/pfpKO male mice display impaired re-adsorption of mammary gland ductules in the last period of fetal life and in the newborn mice. Mammary cancer develops from rudimental ductules expressing the ErbB-2 oncogene and persisting in adult BALB-neuT/pfpKO male mice. Present data show that pfp-mediated mechanisms play crucial roles in mammary gland morphogenesis and in the immunosurveillance of ErbB-2+ carcinomas. Altered re-adsorption of mammary ductules allows ErbB-2+ cells to persist in adult male mice and give rise to carcinomas.  

Attività di ricerca

Immunosurveillance and cancer; Cancer Vaccination

 

POSTER PRESENTATIONS

  • Congress ESCII October 9th-11th 2008 Athen – Greece

PERFORIN-DEPENDENT IMMUNE SURVEILLANCE AGAINST Her-2/neu CARCINOMAS IN TRANSGENIC MALE MICE

Marco Macagno1, Simona Rolla1, Manuela Iezzi2, Irene Merighi1, Federica Cavallo1, Mark J. Smyth3 and Guido Forni1

1Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy, 2Aging Research Centre, G. d’Annunzio University Foundation, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia.

  •  Congress SIC November 23-26 2009 Sesto S. Giovanni (MI) – Italy

NATURAL IMMUNE SURVEILLANCE MECHANISMS DEPENDING ON PERFORIN ARE NATURALLY ABLE TO HAMPER INDOLENT HER-2/NEU MAMMARY CARCINOGENESIS

Marco Macagno1, Simona Rolla1, Manuela Iezzi2, Irene Merighi1, Federica Cavallo1, Mark J. Smyth3 and Guido Forni1

1Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy, 2Aging Research Centre, G. d’Annunzio University Foundation, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

  • Congress EACR-21 June 26-29 2010 Oslo – Norway

INDOLEAMINE 2,3-DIOXYGENASE (IDO) SILENCING FOR IMPROVED ANTITUMOR VACCINATION

Marco Macagno1, Elisabetta Bolli1, Cristina Marchini3, Augusto Amici3, Chiara Riganti2, Amalia Bosia2, Guido Forni1, and Federica Cavallo1

1Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy. 2Department of Genetics, Biology and Biochemistry Laboratory, University of Torino, Torino, Italy. 3Genetic Immunization Laboratory, Department of Molecular Cellular and Animal Biology, University of Camerino, Italy.

  • First International Michelangelo Congress 30th June–1st July 2011 Fondazione Cariplo (MI) Italy

OLIGONUCLEOTIDE-BASED INHIBITION OF THE PRO-ANGIOGENIC ACTIVITY OF HRP-3 IN NEUROBLASTOMA

Poster Presented by Macagno M. 1 Pastorino F.2, Dewilde S.1, Loi M.2, Cilli M.3, Sapio R.1, Barone D.1, Ponzoni M.2

                                 1Bionucleon Srl, Coloretto Giacosa (TO), Italy; 2 Experimental Therapies Unit, Laboratory of Oncology, G. Gaslini Children’s Hospital, Genoa, Italy; 3Animal Research Facility, National Cancer Institute, Genoa, Italy.

  • 2011 Joint Annual Meeting SIICA-DGfI held at Conference Center Palariccione , Riccione  from September 28th to October 1st 2011

PERFORIN-DEPENDENT IMMUNE SURVEILLANCE MITIGATES THE ONSET OF ERBB2 MAMMARY CARCINOMAS IN MALE MICE

Marco Macagno1, Silvio Bandini1, Simona Rolla1, Manuela Iezzi2, Irene Merighi1, Mark J. Smyth3, Guido Forni1 and Federica Cavallo1

1Department of Clinical and Biological Sciences, University of Torino, Italy. 2Aging Research Centre, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia.

  • 53rd Meeting fo the Italian Cancer Society held in Turin from 19th to 22th October 2011

HER-2/NEU MAMMARY TUMORS: THE ONSET IN FEMALE AND IN MALE MICE IS CONTROLLED BY PERFORIN MEDIATED MECHANISMS

Marco Macagno1, Silvio Bandini1, Simona Rolla1, Manuela Iezzi2, Mark J. Smyth3, Guido Forni1 and Federica Cavallo1

1Department of Clinical and Biological Sciences, University of Torino, Italy. 2Aging Research Centre, G. D'Annunzio University Fundation, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Center, East Melbourne, Victoria, Australia.

ORAL PRESENTATIONS

  • 2nd European Congress of Immunology (ECI and EFIS) September 13-16 2009

Berlin – German

ROLE OF PERFORIN-DEPENDENT CYTOTOXICITY IN IMMUNOSURVEILLANCE TO ERBB2 CARCINOGENESIS IN BALB-NEUT TRANSGENIC MICE

Marco Macagno1, Simona Rolla1, Manuela Iezzi2, Irene Merighi1, Federica Cavallo1, Mark J. Smyth3 and Guido Forni1

1Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy, 2Aging Research Centre, G. d’Annunzio University Foundation, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

 

  • Progress In Vaccination Against Cancer PIVAC-10 (EACR) September 27-30 2010 Cambridge – UK

PERFORIN-DEPENDENT IMMUNOSURVEILLANCE HAMPERS THE ONSET OF ERBB-2(NEU)+ MAMMARY CARCINOMAS IN TRANSGENIC MICE

Marco Macagno1, Silvio Bandini1, Simona Rolla1, Manuela Iezzi2, Irene Merighi1, Mark J. Smyth3, Guido Forni1 and Federica Cavallo1

1Department of Clinical and Biological Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy, 2Aging Research Centre, G. d’Annunzio University Foundation, Chieti, Italy. 3Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

 

Congresses 2009

- 7a Giornata di Studio sulle Cellule Staminali (UniStem) held on May 13th 2009, Milan - Italy; 

- 2nd European Congress of Immunology (EFIS-ECI) held on Sep 13th to 16th 2009, Berlin - Germany;

- Symposium “New perspective in tumor immunology” held on Oct 16th 2009, Milan – Italy;

- Doctorate Day 2009 held on Oct 28th 2009, care of CeRMS (Centro di Ricerca in Medicina Sperimentale) Torino – Italy;

- 51st Annual Meeting of the Italian Cancer Society “Cancer research in the technological post-industrial era” held on Nov. 23th to 26th2009, Milan – Italy.

Congresses 2010

- 21st Meeting of the European Association of Cancer Research (EACR-21), held on June 26th to 29 th 2010, Oslo - Norway;

- Progress In Vaccination Against Cancer (PIVAC-10) –EACR-  held on September 27th to 30th, Cambridge – United Kingdom;

- Doctorate Day 2010 held on Nov 12th 2010, care of CeRMS (Centro di Ricerca in Medicina Sperimentale) Torino – Italy;

- MetaFight Workshop held on Dec 2-3th 2010, Molecular Biotecnology Center, Torino - Italy.

 

Congresses 2011

- GIORNATA MAURIZIANA DI EMATOLOGIA held in Torino 1st april 2011;

- The Biology of Cancer Oncogene and Microeviroment held in Torino on may 29th2011; Primary cells, cell culture and mycoplasma prevention held in Torino june 21th 2011;

- First International Michelangelo Congress “Promises and challenges of developing new drugs in oncology” held on 30th June–1st July 2011 Fondazione Cariplo (MI) Italy;

- GENE VACCINATION IN CANCER held in Ascoli Piceno on 15th-17thSeptember 2011;

- EFIS/EJI Course Basic and Translational Immunology “The Innate Immunity” held at Conference Center Palariccione , Riccione – September 27th – 28th 2011;

- 2011 Joint Annual Meeting SIICA-DGfI held at Conference Center Palariccione , Riccione  from September 28th to October 1st 2011;

- 53rd Meeting fo the Italian Cancer Society “BACK TO THE FUTURE Translating cancer research from bedside to bench and back” held in Turin from 19th to 22th October 2011.

 

 

 

From march 2010 to now: stage at Bionucleon Srl (Eporgen-Venture SpA - Bio Industry Park – Colleretto Giacosa (Ivrea), Torino).

 

- International Joint PhD Course in Molecular Oncology, Experimental Immunology and New Drugs Development: Biological Cancer Therapy” (Univ. of Catanzaro, Univ. of Friburgo, Karolinska Institut of Stoccolma and Univ. of Turin) Università degli Studi di Catanzaro “Magna Grecia”, Campus Universitario “S.Venuta”- Catanzaro, Italy 15-19.11.2010.

- SUMMER SCHOOL ON MEDICAL TECHNOLOGIES 2011 “Industrial challenges and process of innovation on medical technologies” held in Grenoble from 18th to 23rd of july 2011

Ultimo aggiornamento: 25/12/2013 23:18
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